Kota Murakami studied at Tokyo College of Music and also trained under the New National Theatre Young Artists Programme. His musical training has also taken him to R. Accademia Filarmonica di Bologna, under the auspices of the Japanese Cultural Affairs Agency. In 2006, he sang as soloist in a performance of Mozart's Coronation Mass at the Bologna University.
He has sung many roles, including Rodolfo in La Bohème, Don Jose in Carmen, Duca di Mantova in Rigoletto, Don Ottavio in Don Giovanni, First Commissioner in Dialogues de Carmélites, Alfred and Blint in Die Fledermaus, Camille in The Merry Widow and A Young Sailor in Tristan und Isolde. In 2008 and 2009, he sang in Strauss’ La Folle by Tokyo Nikikai Opera Foundation directed by Joel Lauwers. He has sung as Rodolfo in La Bohème and Tamino in Die Zauberflöte with the Singapore Lyric Opera.
His subsequent appearances with Tokyo Nikikai Opera Foundation were in Die Zauberflöte and Turandot in July 2011. Kota Murakami is regarded as one of primo uomos of the future.
He is a member of Nikikai, a Japanese organisation of over 2,000 operatic singers.